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Article

Systematic analysis of Zn2Cys6 transcription factors required for development and pathogenicity by high-throughput gene knockout in the rice blast fungus.

PLoS pathogens - 1 Oct 2014

Lu Jianping, Cao Huijuan, Zhang Lilin, Huang Pengyun, Lin Fucheng

Abstract excerpt

Because of great challenges and workload in deleting genes on a large scale, the functions of most genes in pathogenic fungi are still unclear. In this study, we developed a high-throughput gene knockout system using a novel yeast-Escherichia-Agrobacterium shuttle vector, pKO1B, in the rice blast fungus Magnaporthe oryzae. Using this method, we deleted 104 fungal-specific Zn(2)Cys(6) transcription factor (TF)...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25299517
DOI: 10.1371/journal.ppat.1004432
PMCID: PMC4192604

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