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Article

Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis.

Organic letters - 12 Dec 2002

Segura Michael J R, Lodeiro Silvia, Meyer Michelle M, Patel Akash J, Matsuda Seiichi P T

Abstract excerpt

[reaction: see text] Cycloartenol synthase cyclizes and rearranges oxidosqualene to the protosteryl cation and then specifically deprotonates from C-19. To identify mutants that deprotonate differently, randomly generated mutant cycloartenol synthases were selected in a yeast lanosterol synthase...

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Identifiers

PMID: 12465912
DOI: 10.1021/ol0269897

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-12465912-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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