Blinded evaluation of DNA extraction and genotyping of stained Cryptosporidium on glass slides.

doi:10.1046/j.1472-765x.2002.01220.x

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Article

Blinded evaluation of DNA extraction and genotyping of stained Cryptosporidium on glass slides.

Letters in applied microbiology - 1 Jan 2002

Amar C F L, Chalmers R M, Elwin K, Tynan P, McLauchlin J

Abstract excerpt

AIMS: A blinded trial was performed on Cryptosporidium genotyping using polymerase chain reaction (PCR)/restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein (COWP) gene between DNA extracted from oocyst suspensions as compared with DNA from fixed and stained faecal smears on glass microscope slides. METHODS AND RESULTS: Sixty-five faecal smears on slides were stained by one...

Topics

Animals
Cryptosporidium
Cryptosporidium parvum
DNA, Protozoan
Feces
Genotype
Humans
Oocysts
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Potassium Permanganate
Protozoan Proteins
Rosaniline Dyes
Single-Blind Method
Staining and Labeling

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PMID: 12460429
DOI: 10.1046/j.1472-765x.2002.01220.x

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