Article
Blinded evaluation of DNA extraction and genotyping of stained Cryptosporidium on glass slides.
Letters in applied microbiology - 1 Jan 2002
Amar C F L, Chalmers R M, Elwin K, Tynan P, McLauchlin J
Abstract excerpt
AIMS: A blinded trial was performed on Cryptosporidium genotyping using polymerase chain reaction (PCR)/restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein (COWP) gene between DNA extracted from oocyst suspensions as compared with DNA from fixed and stained faecal smears on glass microscope slides. METHODS AND RESULTS: Sixty-five faecal smears on slides were stained by one...
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