Article
Construction of a deep-rough mutant of Burkholderia cepacia ATCC 25416 and characterization of its chemical and biological properties.
The Journal of biological chemistry - 17 Jan 2003
Gronow Sabine, Noah Christian, Blumenthal Antje, Lindner Buko, Brade Helmut
Abstract excerpt
Burkholderia cepacia is a bacterium with increasing importance as a pathogen in patients with cystic fibrosis. The deep-rough mutant Ko2b was generated from B. cepacia type strain ATCC 25416 by insertion of a kanamycin resistance cassette into the gene waaC encoding heptosyltransferase I. Mass sp...
Topics
- Base Sequence
- Burkholderia cepacia
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Glycosyltransferases
- Humans
- Macrophages
- Molecular Sequence Data
- Mutation
- Oligosaccharides
- Spectrometry, Mass, Electrospray Ionization
- Transferases
- Tumor Necrosis Factor-alpha