Article
Structural Analysis of β-Fructofuranosidase from Xanthophyllomyces dendrorhous Reveals Unique Features and the Crucial Role of N-Glycosylation in Oligomerization and Activity.
The Journal of biological chemistry - 25 Mar 2016
Ramírez-Escudero Mercedes, Gimeno-Pérez María, González Beatriz, Linde Dolores, Merdzo Zoran, Fernández-Lobato María, Sanz-Aparicio Julia
Abstract excerpt
Xanthophyllomyces dendrorhousβ-fructofuranosidase (XdINV)is a highly glycosylated dimeric enzyme that hydrolyzes sucrose and releases fructose from various fructooligosaccharides (FOS) and fructans. It also catalyzes the synthesis of FOS, prebiotics that stimulate the growth of beneficial bacteria in human gut. In contrast to most fructosylating enzymes, XdINV produces neo-FOS, which makes it an interesting...
Topics
- Amino Acid Sequence
- Basidiomycota
- Biocatalysis
- Catalytic Domain
- Cloning, Molecular
- Crystallography, X-Ray
- Fructans
- Fructose
- Fungal Proteins
- Gene Expression