Article
Strain typing in Leishmania donovani by using sequence-confirmed amplified region analysis.
International journal for parasitology - 1 Sept 2002
Lewin Sylke, Schönian Gabriele, El Tai Nahla, Oskam Linda, Bastien Patrick, Presber Wolfgang
Abstract excerpt
To differentiate strains of Leishmania donovani, allelic markers at the DNA level were developed by sequence-confirmed amplified region analysis (SCAR). Homologous fragments from different strains of L. donovani were amplified by PCR using random primers and subsequently screened for single-strand conformation polymorphisms. Direct sequencing revealed 55 sequence polymorphisms in eight co-dominant DNA markers; 38...
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