Insufficient expression of the ilv-leu operon encoding enzymes of branched-chain amino acid biosynthesis limits growth of a Bacillus subtilis ccpA mutant.

Abstract excerpt

Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-chain amino acids...

Topics

• Amino Acids, Branched-Chain
• Bacillus subtilis
• Bacterial Proteins
• Culture Media
• DNA-Binding Proteins
• Gene Expression Regulation, Bacterial
• Glucose
• Glutamate Synthase
• Mutation
• Operon
• Promoter Regions, Genetic
• Repressor Proteins
• Transcription, Genetic