Article
Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum.
Applied and environmental microbiology - 1 Aug 2002
Rochelle Paul A, Marshall Marilyn M, Mead Jan R, Johnson Anne M, Korich Dick G, Rosen Jeffrey S, De Leon Ricardo
Abstract excerpt
In vitro cell cultures were compared to neonatal mice for measuring the infectivity of five genotype 2 isolates of Cryptosporidium parvum. Oocyst doses were enumerated by flow cytometry and delivered to animals and cell monolayers by using standardized procedures. Each dose of oocysts was inoculated into up to nine replicates of 9 to 12 mice or 6 to 10 cell culture wells. Infections were detected by hematoxylin...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
