Article
Functional replacement of the tobacco rattle virus cysteine-rich protein by pathogenicity proteins from unrelated plant viruses.
Virology - 5 Jul 2002
Liu Hui, Reavy Brian, Swanson Maud, MacFarlane Stuart A
Abstract excerpt
Mutation of the 16K gene encoded by RNA1 of Tobacco rattle virus (TRV) greatly reduced the levels of viral RNA that accumulated in both infected protoplasts and plants, showing that the 16K cysteine-rich protein (CRP) is required for efficient multiplication of TRV. Overexpression of the 16K protein, either from an additional copy of the gene carried on TRV RNA2 or from a PVX vector, led to an increase in the...
Topics
- Cysteine
- Gene Silencing
- Genetic Vectors
- Molecular Weight
- Mutation
- Plant Viruses
- RNA Viruses
- Reassortant Viruses
- Suppression, Genetic
- Viral Proteins