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Article

Characterisation of genetically modified cucumber mosaic virus expressing histidine-tagged 1a and 2a proteins.

Archives of virology - 1 Jan 2000

Gal-On A, Canto T, Palukaitis P

Abstract excerpt

Biological active cDNA clones of cucumber mosaic virus (CMV) RNAs 1 and 2 were modified by addition of sequences that encode hexahistidine (His-tag) at the amino- (N-) or carboxy- (C-) terminus of the 1a and 2a proteins. These proteins are essential components of the viral RNA-dependent RNA polymerase (RdRp). In all but one case, addition of the His-tag did not significantly affect the yields of the corresponding...

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Identifiers

PMID: 10664404
DOI: 10.1007/s007050050003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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