Article
Vibrational modes of tyrosines in cytochrome c oxidase from Paracoccus denitrificans: FTIR and electrochemical studies on Tyr-D4-labeled and on Tyr280His and Tyr35Phe mutant enzymes.
Biochemistry - 23 Jul 2002
Hellwig Petra, Pfitzner Ute, Behr Julia, Rost Borries, Pesavento Russell P, Donk Wilfred V, Gennis Robert B, Michel Hartmut, Ludwig Bernd, Mäntele Werner
Abstract excerpt
A combined electrochemical and FTIR spectroscopic approach was used to identify the vibrational modes of tyrosines in cytochrome c oxidase from Paracoccus denitrificans which change upon electron transfer and coupled proton transfer. Electrochemically induced FTIR difference spectra of the Tyr-D4-labeled cytochrome c oxidase reveal that only small contributions arise from the tyrosines. Contributions between 1600...
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