Differential spectrum of mutations that activate the Escherichia coli bgl operon in an rpoS genetic background.

Abstract excerpt

The bgl promoter is silent in wild-type Escherichia coli under standard laboratory conditions, and as a result, cells exhibit a beta-glucoside-negative (Bgl-) phenotype. Silencing is brought about by negative elements that flank the promoter and include DNA structural elements and sequences that interact with the nucleoid-associated protein H-NS. Mutations that confer a Bgl+ phenotype arise spontaneously at a...

Topics

• Bacterial Proteins
• DNA Transposable Elements
• Escherichia coli
• Glucosides
• Mutation
• Operon
• Sigma Factor