Article
Crystallization of a stringent response factor from Aquifex aeolicus.
Acta crystallographica. Section D, Biological crystallography - 1 Jul 2002
Kristensen Ole, Laurberg Martin, Gajhede Michael
Abstract excerpt
The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein...
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