Article
Endocytic intermediates involved with the intracellular trafficking of a fluorescent cellular prion protein.
The Journal of biological chemistry - 6 Sept 2002
Magalhães Ana C, Silva Juliana A, Lee Kil S, Martins Vilma R, Prado Vania F, Ferguson Stephen S G, Gomez Marcus V, Brentani Ricardo R, Prado Marco A M
Abstract excerpt
We have investigated the intracellular traffic of PrP(c), a glycosylphosphatidylinositol (GPI)-anchored protein implicated in spongiform encephalopathies. A fluorescent functional green fluorescent protein (GFP)-tagged version of PrP(c) is found at the cell surface and in intracellular compartments in SN56 cells. Confocal microscopy and organelle-specific markers suggest that the protein is found in both the...
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