Article
Fluorescent-BOX-PCR for resolving bacterial genetic diversity, endemism and biogeography.
BMC microbiology - 15 Dec 2008
Brusetti Lorenzo, Malkhazova Iana, Gtari Maher, Tamagnini Isabella, Borin Sara, Merabishvili Maya, Chanishvili Nina, Mora Diego, Cappitelli Francesca, Daffonchio Daniele
Abstract excerpt
BACKGROUND: BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) is one of the most used techniques in biogeography studies of microbial isolates. However the traditional separation of BOX-PCR patterns by agarose gel electrophoresis suffers many limitations. The aim of this research was to set up a fluorescent BOX-PCR (F-BOX-PCR) assay in which separation of PCR products is automated in a capillary...
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