Article
Real-Time PCR for Detection and Quantitation of <i>Leishmania</i> in Mouse Tissues
1 May 2002
Abstract excerpt
Leishmania spp. are intracellular protozoan parasites that cause a wide spectrum of diseases in humans and dogs worldwide. However, monitoring of the Leishmania burden in its different hosts is still based on cumbersome and poorly sensitive methods. Here we have developed a highly accurate real-time PCR assay with which to reproducibly detect and quantify the relative Leishmania major burden in mouse tissue...
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