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Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites

1 Jan 2020

Abstract excerpt

Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique presented here is sequence-independent and is well detailed in the...

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PMID: 33659564
DOI: 10.21769/bioprotoc.3598

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