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Article

Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality

1 May 2002

Abstract excerpt

BACKGROUND: The aim of the present study was to define the effect of apoptosis on sperm quality and function. METHODS: The apoptotic features in sperm were assessed in 60 subfertile subjects, using Annexin-V staining for phosphatidylserine (PS) externalization and Tdt-mediated dUTP nick end labelling (TUNEL) assay for DNA fragmentation. RESULTS: On average, about 45% of the sperm were found to be apoptotic based...

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PMID: 11980750
DOI: 10.1093/humrep/17.5.1266

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