Article
Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients.
Current medicinal chemistry - 1 Apr 2002
Boór K, Rónai Z, Nemoda Z, Gaszner P, Sasvári-Székely M, Guttman A, Kalász H
Abstract excerpt
A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic...
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