Article
A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomycescerevisiae.
Proceedings of the National Academy of Sciences of the United States of America - 16 Apr 2002
Feng Wenqin, Hopper Anita K
Abstract excerpt
Los1p, the Saccharomyces cerevisiae exportin-t homologue, binds tRNA and functions in pre-tRNA splicing and export of mature tRNA from the nucleus to the cytosol. Because LOS1 is unessential in yeast, other pathways for tRNA nuclear export must exist. We report that Cca1p, which adds nucleotides C, C, and A to the 3' end of tRNAs, is a multicopy suppressor of the defect in tRNA nuclear export caused by los1 null...
Topics
- Active Transport, Cell Nucleus
- Cell Nucleus
- Cytosol
- DNA
- Escherichia coli
- Fungal Proteins
- Green Fluorescent Proteins
- In Situ Hybridization, Fluorescence
- Luminescent Proteins
- Microscopy, Fluorescence
- Models, Biological
- Mutation
- Nuclear Pore Complex Proteins
- Plasmids