Article
A composite hormone response element regulates transcription of the rat GHRH receptor gene.
Endocrinology - 1 Apr 2002
Nogami Haruo, Hiraoka Yoshiki, Matsubara Maki, Nonobe Eriko, Harigaya Toshio, Katayama Masateru, Hemmi Noriaki, Kobayashi Shuzo, Mogi Koichi, Aiso Sadakazu, Kawamura Koki, Hisano Setsuji
Abstract excerpt
To further elucidate the molecular mechanisms underlying the transcriptional regulation of the GHRH receptor (GHRH-R) gene, hormonal regulation of the promoter activity of this gene was examined. An approximately 3-kb genomic fragment spanning the promoter region of the gene was sequenced and the transcription start site was determined by RT-PCR and RNase protection assay. A major start site was localized at -105...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- Codon
- Gene Expression Regulation
- Genes, Reporter
- Glucocorticoids
- Immunoenzyme Techniques
- Molecular Sequence Data
- Mutation
- Plasmids
- Rats
- Receptors, LHRH