Article
Hierarchical high-throughput SNP genotyping of the human Y chromosome using MALDI-TOF mass spectrometry.
Nucleic acids research - 15 Mar 2002
Paracchini Silvia, Arredi Barbara, Chalk Rod, Tyler-Smith Chris
Abstract excerpt
We have established the use of a primer extension/mass spectrometry method (the PinPoint assay) for high-throughput SNP genotyping of the human Y chromosome. 118 markers were used to define 116 haplogroups and typing was organised in a hierarchical fashion. Twenty multiplex PCR/primer extension reactions were set up and each sample could be assigned to a haplogroup with only two to five of these multiplex...
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