Article
Allelic exchange mutagenesis of rpoN encoding RNA-polymerase sigma54 subunit in Helicobacter pylori.
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy - 1 Sept 2001
Fujinaga R, Nakazawa T, Shirai M
Abstract excerpt
The rpoN gene, encoding the alternative sigma factor (sigma54) of Helicobacter pylori, was amplified from genomic DNA. H. pylori rpoN has an overall similarity to the rpoN of other bacteria, but lacks a glutamine (Q)-rich region in region I and an acidic region in region II. When the rpoN gene was disrupted, the mutant was found to be completely nonmotile. Because the flaB gene has an rpoN consensus sequence in...
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