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Article

Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography.

Nucleic acids research - 1 Feb 2002

Deng Dajun, Deng Guoren, Smith Michael F, Zhou Jing, Xin Huijun, Powell Steven M, Lu Youyong

Abstract excerpt

We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used in confirmation studies. Consistent with the BstUI digestion assay, the 294 bp PCR product of the modified hMLH1...

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Identifiers

PMID: 11809901
DOI: 10.1093/nar/30.3.e13
PMCID: PMC100312

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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