Quantitative real-time PCR using TaqMan and SYBR Green for<i>Actinobacillus actinomycetemcomitans</i>,<i>Porphyromonas gingivalis</i>,<i>Prevotella intermedia</i>,<i>tetQ</i>gene and total bacteria

Abstract excerpt

Accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis of periodontal diseases. The present study was designed to assess the sensitivity, specificity and quantitativity of the real-time PCR using the GeneAmp Sequence Detection System with two fluorescence chemistries. TaqMan probe with reporter and quencher dye, and SYBR Green dye were used for sources of the...

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