Prevalence and Genetic Profiling of Virulence Determinants of Non-O157 Shiga Toxin-Producing<i>Escherichia coli</i>Isolated from Cattle, Beef, and Humans, Calcutta, India

Abstract excerpt

We investigated the prevalence of Shiga toxin-producing Escherichia coli (STEC) in hospitalized diarrhea patients in Calcutta, India, as well as in healthy domestic cattle and raw beef samples collected from the city's abattoir. Multiplex polymerase chain reaction using primers specific for stx1 and stx2 detected STEC in 18% of cow stool samples, 50% of raw beef samples, and 1.4% and 0.6% of bloody and watery...

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