Article
[Studies on the properties of dendritic cells from cord blood CD34+ cells and peripheral blood monocytes].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Nov 1999
Wang X, Pei X, Li L
Abstract excerpt
OBJECTIVE: Ex vivo expansion and purification of dendritic cells(DC) with typical phenotype, morphology and function are critical to the further studies on DC and their clinical application. METHODS: CD34+ cells were isolated from umbilical cord blood by using a high-gradient magnetic cell sorting system (MACS), and peripheral blood mononuclear cells (PBMC) were depleted of T and B cells by mixing them with T/CD2...
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