Article
Histidine 90 function in 4-chlorobenzoyl-coenzyme a dehalogenase catalysis.
Biochemistry - 13 Nov 2001
Zhang W, Wei Y, Luo L, Taylor K L, Yang G, Dunaway-Mariano D, Benning M M, Holden H M
Abstract excerpt
4-chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of 4-CBA-CoA by attack of Asp145 on the C4 of the substrate benzoyl ring to form a Meisenheimer intermediate (EMc), followed by expulsion of chloride ion to form an arylated enzyme intermediate (EAr) and, finally, ester hydrolysis in EAr to form 4-hydroxybenzoyl-CoA (4-HBA-CoA). This study examines the contribution of the...
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