Article
The strength of dehalogenase-substrate hydrogen bonding correlates with the rate of Meisenheimer intermediate formation.
Biochemistry - 12 Aug 2003
Dong Jian, Lu Xuefeng, Wei Yansheng, Luo Lusong, Dunaway-Mariano Debra, Carey Paul R
Abstract excerpt
4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of 4-CBA-CoA to 4-hydroxybenzoyl-CoA by using an active site aspartate as the nucleophile. Formation of the corresponding Meisenheimer complex (EMc) is followed by chloride ion expulsion which forms the arylated intermediate (EAr). This is then hydrolyzed to the product. In this paper, we explore the relationship between...
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