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Simultaneous Detection of<i>Chlamydophila pneumoniae</i>and<i>Mycoplasma pneumoniae</i>by Use of Molecular Beacons in a Duplex Real-Time PCR

20 Dec 2007

Abstract excerpt

A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumoniae and the ompA gene for C. pneumoniae, was compared with two conventional PCR assays targeting the 16S rRNA gene and...

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PMID: 18094125
DOI: 10.1128/jcm.01540-07

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