Evaluation and Improvement of Real-Time PCR Assays Targeting <i>lytA</i> , <i>ply</i> , and <i>psaA</i> Genes for Detection of Pneumococcal DNA

Abstract excerpt

The accurate diagnosis of pneumococcal disease has frequently been hampered not only by the difficulties in obtaining isolates of the organism from patient specimens but also by the misidentification of pneumococcus-like viridans group streptococci (P-LVS) as Streptococcus pneumoniae. This is especially critical when the specimen comes from the respiratory tract. In this study, three novel real-time PCR assays...

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