Article
Insertion or deletion of the Cheo box modifies radiation inducibility of Clostridium promoters.
Applied and environmental microbiology - 1 Oct 2001
Nuyts S, Van Mellaert L, Barbé S, Lammertyn E, Theys J, Landuyt W, Bosmans E, Lambin P, Anné J
Abstract excerpt
Radiation-inducible promoters are being used in many viral vector systems to obtain spatial and temporal control of gene expression. It was previously proven that radiation-induced gene expression can also be obtained in a bacterial vector system using anaerobic apathogenic clostridia. The effect of radiation inducibility was detected using mouse tumor necrosis factor alpha (mTNF-alpha) as a model protein under...
Topics
- Animals
- Base Sequence
- Cellulase
- Clostridium
- Gene Deletion
- Gene Expression Regulation, Bacterial
- Mice
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic
- Promoter Regions, Genetic
- Rec A Recombinases
- Reverse Transcriptase Polymerase Chain Reaction