Article
Use of a genetic marker for wild-type potentially pathogenic infectious bursal disease viruses.
Avian diseases - 1 Jan 2000
Jackwood D J, Byerley E H, Sommer S E
Abstract excerpt
An amino acid mutation at residue 284 (Ala to Thr) in the VP2 protein of infectious bursal disease viruses (IBDVs) has been correlated with the ability to replicate in cell culture. In this study, we designed a molecular test for this mutation. The reverse transcriptase/polymerase chain reaction (RT/PCR) was used to amplify a 743-bp region of the VP2 gene that contained the codon for amino acid 284. The...
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