Article
A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis.
Biochimica et biophysica acta - 29 Jun 2001
Storey M K, Wu W I, Voelker D R
Abstract excerpt
A genetic screen for ethanolamine auxotrophs has identified a novel mutant allele of the morphogenesis checkpoint dependent (MCD)-4 gene, designated mcd4-P301L. In the presence of a null allele for the phosphatidylserine (PtdSer) decarboxylase 1 gene (psd1 Delta), the mcd4-P301L mutation confers temperature sensitivity for growth on minimal medium. This growth defect is reversed by either ethanolamine or choline...
Topics
- Ethanolamine
- Fungal Proteins
- Glycosylphosphatidylinositols
- Membrane Proteins
- Mutation
- Phospholipids
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Temperature