Article
DNA repair and sequence context affect (1)O(2)-induced mutagenesis in bacteria.
Nucleic acids research - 1 Jul 2001
Agnez-Lima L F, Napolitano R L, Fuchs R P, Mascio P D, Muotri A R, Menck C F
Abstract excerpt
Electronic excited molecular oxygen (singlet oxygen, (1)O(2)) is known to damage DNA, yielding mutations. In this work, the mutagenicity induced by (1)O(2) in a defined sequence of DNA was investigated after replication in Escherichia coli mutants deficient for nucleotide and base excision DNA repair pathways. For this purpose a plasmid containing a (1)O(2)-damaged 14 base oligonucleotide was introduced into...
Topics
- Bacterial Proteins
- Base Sequence
- DNA Damage
- DNA Glycosylases
- DNA Repair
- DNA Replication
- DNA-Formamidopyrimidine Glycosylase
- Endodeoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Genetic Vectors
- Mutagenesis
- Mutation
- N-Glycosyl Hydrolases