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Article

Nuclear Transfer Protocol Affects Messenger RNA Expression Patterns in Cloned Bovine Blastocysts

1 Jul 2001

Abstract excerpt

The successful production of embryos by nuclear transfer (NT) employing cultured somatic donor cells depends upon a variety of factors. The objective of the present study was to investigate the effects 1) of two different activation protocols, 2) the use of quiescent or nonquiescent donor cells (G(0) or G(1) of the cell cycle), and 3) passage number of donor cells on the relative abundance (RA) of eight specific...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 11420254
DOI: 10.1095/biolreprod65.1.309

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