Article
The use of recombinant baculoviruses for sustained expression of human cytomegalovirus immediate early proteins in fibroblasts.
Virology - 5 Jun 2001
Dwarakanath R S, Clark C L, McElroy A K, Spector D H
Abstract excerpt
The isolation of viruses with mutations in essential genes requires that they be propagated in cells expressing the wild-type proteins. This has been a particularly challenging problem for studying mutations in the human cytomegalovirus (HCMV) immediate early (IE) gene, IE2 86. In the past, we tried a number of approaches to derive human fibroblasts expressing wild-type IE2 86, but were unable to maintain...
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