Differentiation of <i>Campylobacter coli</i> , <i>Campylobacter jejuni</i> , <i>Campylobacter lari</i> , and <i>Campylobacter upsaliensis</i> by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene <i>lpxA</i>

Abstract excerpt

We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in Escherichia coli strain SM105...

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