New Tests for Syphilis: Rational Design of a PCR Method for Detection of <i>Treponema pallidum</i> in Clinical Specimens Using Unique Regions of the DNA Polymerase I Gene

Abstract excerpt

A sensitive and specific PCR method to detect Treponema pallidum in clinical specimens was developed. PCR primers were designed based on two unique features of the DNA polymerase I gene (polA). The first distinctive characteristic is that the region codes for a high cysteine content and has low homology with similar regions of DNA polymerase I gene from known microorganisms. The second unique feature is the...

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