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Article

Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge.

Journal of clinical microbiology - 1 May 2001

Schroeder J M, Booton G C, Hay J, Niszl I A, Seal D V, Markus M B, Fuerst P A, Byers T J

Abstract excerpt

This study identified subgenic PCR amplimers from 18S rDNA that were (i) highly specific for the genus Acanthamoeba, (ii) obtainable from all known genotypes, and (iii) useful for identification of individual genotypes. A 423- to 551-bp Acanthamoeba-specific amplimer ASA.S1 obtained with primers JDP1 and JDP2 was the most reliable for purposes i and ii. A variable region within this amplimer also identified...

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Identifiers

PMID: 11326011
DOI: 10.1128/JCM.39.5.1903-1911.2001
PMCID: PMC88046

Genes

JDP2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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