Article
Identification of 18S ribosomal DNA genotype of Acanthamoeba from patients with keratitis in North China.
Investigative ophthalmology & visual science - 1 Jun 2004
Zhang Yan, Sun Xuguang, Wang Zhiqun, Li Ran, Luo Shiyun, Jin Xiuying, Deng Shijing, Chen Wei
Abstract excerpt
PURPOSE: To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) of the Acanthamoeba strains isolated from patients with keratitis in northern China. METHODS: The genus-specific primers JDP1 and JDP2 were used for the amplification of the amplimer ASA.S1. With DNA PCR and sequencing, Rns genotypes were identified according to the DF3 sequence variances. RESULTS: Of all 26 DF3 sequences obtained from...
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