Article
The dissociation rate of estrogen receptor alpha from the consensus estrogen response element.
Molecular and cellular endocrinology - 25 Apr 2001
Szatkowski Ozers M, Hill J J, Ervin K, Royer C A, Gorski J
Abstract excerpt
The rate of dissociation of recombinant, purified human estrogen receptor alpha (ERalpha) from a fluorescein-labeled DNA containing the consensus vitellogenin ERE sequence (F-vitERE) was determined in real time using fluorescence anisotropy. The complex of estradiol-occupied ERalpha with F-vitERE had an apparent dissociation rate of 1.48+/-0.06x10(-2) s(-1) and a half-life of 46.6 s at room temperature. The...
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