Article
Functional studies of the bacterial avirulence protein AvrPto by mutational analysis.
Molecular plant-microbe interactions : MPMI - 1 Apr 2001
Chang J H, Tobias C M, Staskawicz B J, Michelmore R W
Abstract excerpt
Pseudomonas syringae pathovars expressing avrPto are avirulent on plants expressing the resistance gene Pto. Over 85 mutants of avrPto were generated with multiple strategies, and several assays were used to characterize AvrPto function. Only a core of 95 amino acids of the 164 residues was sufficient for binding Pto in the yeast two-hybrid system. Only nine of 65 mutant proteins of AvrPto with amino acid...
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