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Article

Functional analysis of the plant disease resistance gene Pto using DNA shuffling.

The Journal of biological chemistry - 17 Jun 2005

Bernal Adriana J, Pan Qilin, Pollack Jeff, Rose Laura, Kozik Alexander, Willits Neil, Luo Yao, Guittet Muriel, Kochetkova Elena, Michelmore Richard W

Abstract excerpt

Pto is a serine/threonine kinase that mediates resistance in tomato to strains of Pseudomonas syringae pv. tomato expressing the (a)virulence proteins AvrPto or AvrPtoB. DNA shuffling was used as a combinatorial in vitro genetic approach to dissect the functional regions of Pto. The Pto gene was shuffled with four of its paralogs from a resistant haplotype to create a library of recombinant products that was...

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Identifiers

PMID: 15790558
DOI: 10.1074/jbc.M500992200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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