Article
Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi.
Microbiology (Reading, England) - 1 May 2001
Arnold Dawn L, Jackson Robert W, Fillingham A Jane, Goss Susan C, Taylor John D, Mansfield John W, Vivian Alan
Abstract excerpt
DNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1) from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with all P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- and avrPpiB-containing fragments, two additional fragments were amplified: one contained a single open reading frame...
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