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PCR-Based Diagnosis of <i>Helicobacter pylori</i> Infection and Real-Time Determination of Clarithromycin Resistance Directly from Human Gastric Biopsy Samples

1 Apr 2001

Abstract excerpt

A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarithromycin resistance was applied directly to antral gastric biopsy samples. Comparison with culture...

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PMID: 11283030
DOI: 10.1128/jcm.39.4.1217-1220.2001

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