Article
Two different combinations of RNA-binding domains determine the RNA binding specificity of nucleolin.
The Journal of biological chemistry - 27 Apr 2001
Ginisty H, Amalric F, Bouvet P
Abstract excerpt
Nucleolin is an abundant nucleolar protein involved in several steps of ribosome biogenesis. The protein is highly conserved through evolution and possesses four RNA-binding domains (RBD), which are likely to determine its RNA binding specificity. Previous studies have shown that nucleolin interacts with two different RNA targets. The first is a small stem-loop structure, the nucleolin recognition element (NRE),...
Topics
- Animals
- Base Sequence
- Conserved Sequence
- Cricetinae
- DNA Mutational Analysis
- Dose-Response Relationship, Drug
- Gene Deletion
- Kinetics
- Molecular Sequence Data
- Mutation
- Phosphoproteins
- Plasmids
- Protein Binding
- Protein Conformation
- Protein Structure, Tertiary
- RNA
- RNA, Ribosomal
- RNA-Binding Proteins