Article
N-linked glycosylation of the HIV type-1 gp120 envelope glycoprotein as a major determinant of CCR5 and CXCR4 coreceptor utilization.
The Journal of biological chemistry - 20 Apr 2001
Pollakis G, Kang S, Kliphuis A, Chalaby M I, Goudsmit J, Paxton W A
Abstract excerpt
The variable V1V2 and V3 regions of the human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein (gp120) can influence viral coreceptor usage. To substantiate this we generated isogenic HIV-1 molecularly cloned viruses that were composed of the HxB2 envelope backbone containing the V1V2 and V3 regions from viruses isolated from a patient progressing to disease. We show that the V3 amino acid charge per...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- CD4-Positive T-Lymphocytes
- Chimera
- Cloning, Molecular
- Glycosylation
- HIV Envelope Protein gp120
- HIV-1
- Humans
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Receptors, CCR5