Article
Delivery of a cyclic adenosine 3',5'-monophosphate response element-binding protein (creb) mutant to seminiferous tubules results in impaired spermatogenesis.
Endocrinology - 1 Feb 2001
Scobey M, Bertera S, Somers J, Watkins S, Zeleznik A, Walker W
Abstract excerpt
FSH binding to Sertoli cells is required for optimal production of sperm in mammals. The cAMP response element-binding protein (CREB) is a major mediator of FSH-induced changes in gene expression. To determine whether CREB is required for spermatogenesis, an adenovirus encoding a phosphorylation-defective CREB mutant (AdCREBm1) was used to inhibit CREB activity in Sertoli cells. Addition of AdCREBm1 to primary...
Topics
- Animals
- Apoptosis
- Cells, Cultured
- Cyclic AMP Response Element-Binding Protein
- Gene Transfer Techniques
- Male
- Mutation
- Rats
- Rats, Sprague-Dawley
- Seminiferous Tubules
- Sertoli Cells
- Spermatocytes
- Spermatogenesis
- Spermatozoa