Article
Simultaneous identification of five bifidobacterium species isolated from human beings using multiple PCR primers.
Systematic and applied microbiology - 1 Oct 2000
Dong X, Cheng G, Jian W
Abstract excerpt
On the basis of 16S rRNA sequences, 5 species-specific forward primers were designed for the identification of 5 Bifidobacterium species isolated from human intestine, namely B. bifidum, B. adolescentis, B. infantis, B. breve and B. longum. As the 5 primers targeted at different sites of 16S rDNA, by using their mixture and a genus-specific reversed primer, the 5 Bifidobacterium species can be simultaneously...
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